Topology Prediction of Membrane Proteins: Why, How and When?

Topology prediction of helical transmembrane proteins: how far have we reached?

Transmembrane protein topology prediction methods play important roles in structural biology, because the structure determination of these types of proteins is extremely difficult by the common biophysical, biochemical and molecular biological methods. The need for accurate prediction methods is high, as the number of known membrane protein structures fall far behind the estimated number of the...

In Silico and in Vitroinvestigations on cry4aand cry11atoxins of Bacillus thuringiensis var Israelensis

In the present study we attempted to correlate the structure and function of the cry11a (72 kDa) and cry4a (135 kDa) proteins of Bacillus thuringiensis var israelensis. Homology modeling and secondary structure predictions were done to locate most probable regions for finding helices or strands in these proteins. The JPRED (JPRED consensus secondary structure prediction server) secondary struct...

Improved membrane protein topology prediction by domain assignments.

Topology predictions for integral membrane proteins can be substantially improved if parts of the protein can be constrained to a given in/out location relative to the membrane using experimental data or other information. Here, we have identified a set of 367 domains in the SMART database that, when found in soluble proteins, have compartment-specific localization of a kind relevant for membra...

The Why, Who, What, How, and When of Patient Engagement in Healthcare Organizations: A Response to Recent Commentaries

Application of qualitative research in management (why, when and how)

Application of qualitative research in management (why, when and how) Qualitative research has been increasingly used in all sciences. In this paper we will discuss about the applications of qualitative research methods in management sciences. The differences between quantitative and qualitative researches will be clarified and the statistical methods which are suitable for such researches will...